Tomato (Solanum lycopersicum) variety discrimination and hybridization analysis based on the 5S rRNA region
نویسندگان
چکیده
The tomato (Solanum lycopersicum) is a major vegetable crop worldwide. To satisfy popular demand, more than 500 tomato varieties have been bred. However, a clear variety identification has not been found. Thorough understanding of the phylogenetic relationship and hybridization information of tomato varieties is very important for further variety breeding. Thus, in this study, we collected 26 tomato varieties and attempted to distinguish them based on the 5S rRNA region, which is widely used in the determination of phylogenetic relations. Sequence analysis of the 5S rRNA region suggested that a large number of nucleotide variations exist among tomato varieties. These variable nucleotide sites were also informative regarding hybridization. Chromas sequencing of Yellow Mountain View and Seuwiteuking varieties indicated three and one variable nucleotide sites in the non-transcribed spacer (NTS) of the 5S rRNA region showing hybridization, respectively. Based on a phylogenetic tree constructed using the 5S rRNA sequences, we observed that 16 tomato varieties were divided into three groups at 95% similarity. Rubiking and Sseommeoking, Lang Selection Procedure and Seuwiteuking, and Acorn Gold and Yellow Mountain View exhibited very high identity with their partners. This work will aid variety authentication and provides a basis for further tomato variety breeding.
منابع مشابه
Variation Revealed by SNP Genotyping and Morphology Provides Insight into the Origin of the Tomato
Tomato, Solanum lycopersicum, is divided into two widely distributed varieties: the cultivated S. lycopersicum var. lycopersicum, and the weedy S. lycopersicum var. cerasiforme. Solanum pimpinellifolium is the most closely related wild species of tomato.The roles of S. pimpinellifolium and S. l. cerasiforme during the domestication of tomato are still under debate. Some authors consider S. l. c...
متن کاملNew solanocapsine-type tomato glycoside from ripe fruit of Solanum lycopersicum.
A new solanocapsine-type tomato glycoside, a novel and interesting natural steroidal glycoside, was isolated from a mini tomato, Solanum lycopersicum L. The chemical structure of the new minor glycoside, esculeoside B-5 (3), was determined to be (5S,22R,23S,24R,25S)-22,26-epimino-16β,23-epoxy-3β,23,24-trihydroxycholestane 3-O-β-lycotetraoside.
متن کاملWorm castings-based growing media with biochar and arbuscular mycorrhizal fungi for producing organic tomato (Solanum lycopersicum L.) in greenhouse.
Organic vegetable production has specific research and innovation requirements which are not shared by other parts of the food and farming sector. A pot experiment was conducted to investigate the interactive effects of few permitted organic inputs such as arbuscular mycorrhizal fungi, biochar, and different ratios of peat:worm casting on tomato (Solanum lycopersicum L.) growth, mycorrhizal dep...
متن کاملInfluence of salicylic and jasmonic acids on the antioxidant systems of tomato (Solanum lycopersicum cv. Superchief) plants under biotic stresses. Sahar Gholi-Tolouie1, Mahdi Davari2, Nemat Sokhandan-Bashir1 and Mohammad Sedghi3*
Changes in the activity of antioxidant enzymes, proline, and total soluble carbohydrates (TSC) were evaluated in tomato (Solanum lycopercicum cv. Superchief) plants in response to salicylic acid (SA) and jasmonic acid (JA) and inoculation with CMV (Cucumber mosaic virus) on days 0, 1, 2, 4, 6, 8, and 15 after virus inoculation (dpi). Results showed that proline content increased in all treatmen...
متن کاملIn Silico Identification and Experimental Validation of Insertion–Deletion Polymorphisms in Tomato Genome
Comparative analysis of the genome sequences of Solanum lycopersicum variety Heinz 1706 and S. pimpinellifolium accession LA 1589 using MUGSY software identified 145 695 insertion-deletion (InDel) polymorphisms. A selected set of 3029 candidate InDels (≥2 bp) across the entire tomato genome were subjected to PCR validation, and 82.4% could be verified. Of 2272 polymorphic InDels between LA 1589...
متن کامل